small cell lung cancer sclc lines h1618 Search Results


human small cell lung cancer sclc lines h1618  (ATCC)
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ATCC human small cell lung cancer sclc lines h1618
Figure 1. Comparison of RLIP76 levels in cultured malignant cells versus nonmalignant cells. Aliquots of crude detergent extracts of the membrane fractions of malignant cells <t>(H1618,</t> H358, OVCAR-3, PC-3, MCF-7, B16, HepG2, and DG-1) and nonmalignant cells (HAVSM, HUVEC, HLMVEC, and HLBEC), containing 200 Ag protein were used for SDS-PAGE and Western blotting against anti-RLIP76 IgG as primary antibody and horseradish peroxidase–conjugated goat anti-rabbit IgG as secondary antibody and developed with 4-chloro-1-napthol as chromogenic substrate. Results were quantified by scanning densitometry of the full-length RLIP76 protein band near 109 kDa. h-Actin was used as an internal control.
Human Small Cell Lung Cancer Sclc Lines H1618, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Comparison of RLIP76 levels in cultured malignant cells versus nonmalignant cells. Aliquots of crude detergent extracts of the membrane fractions of malignant cells (H1618, H358, OVCAR-3, PC-3, MCF-7, B16, HepG2, and DG-1) and nonmalignant cells (HAVSM, HUVEC, HLMVEC, and HLBEC), containing 200 Ag protein were used for SDS-PAGE and Western blotting against anti-RLIP76 IgG as primary antibody and horseradish peroxidase–conjugated goat anti-rabbit IgG as secondary antibody and developed with 4-chloro-1-napthol as chromogenic substrate. Results were quantified by scanning densitometry of the full-length RLIP76 protein band near 109 kDa. h-Actin was used as an internal control.

Journal: Cancer Research

Article Title: Regression of Melanoma in a Murine Model by RLIP76 Depletion

doi: 10.1158/0008-5472.can-05-3534

Figure Lengend Snippet: Figure 1. Comparison of RLIP76 levels in cultured malignant cells versus nonmalignant cells. Aliquots of crude detergent extracts of the membrane fractions of malignant cells (H1618, H358, OVCAR-3, PC-3, MCF-7, B16, HepG2, and DG-1) and nonmalignant cells (HAVSM, HUVEC, HLMVEC, and HLBEC), containing 200 Ag protein were used for SDS-PAGE and Western blotting against anti-RLIP76 IgG as primary antibody and horseradish peroxidase–conjugated goat anti-rabbit IgG as secondary antibody and developed with 4-chloro-1-napthol as chromogenic substrate. Results were quantified by scanning densitometry of the full-length RLIP76 protein band near 109 kDa. h-Actin was used as an internal control.

Article Snippet: Human small cell lung cancer (SCLC) lines H1618 and non–small cell lung cancer (NSCLC) lines H358 (bronchioalveolar), PC-3 (human prostate), and B16 mouse skin melanoma were purchased from American Type Culture Collection (Manassas, VA).

Techniques: Comparison, Cell Culture, Membrane, SDS Page, Western Blot, Control